Anti-ZFAT Antibody, clone Z4

Zinc finger protein ZFAT (UniProt: Q7TS63; also known as Zinc finger protein 406) is encoded by the Zfat (also known as Gm922, Zfat1, Zfp406, Znf406) gene (Gene ID: 380993) in mouse. ZFAT is a transcription factor and is suggested to be involved in lymphocyte signaling. Human homolog ZFAT (also known as zinc finger gene in AITD susceptibility region) is strongly expressed in placenta, spleen, kidney, testis and peripheral blood leukocytes. It is also expressed in CD4+ and CD8+ T-cells, CD19+ B-cells and CD14+ monocytes. ZFAT is primarily localized in the nucleus, where it functions as a transcription factor, essential for regulating gene expression and is also found in the cytosol. Zinc finger protein ZFAT contains multiple zinc finger motifs that facilitate its interaction with DNA, enabling specific binding to target gene sequences. Four isoforms of ZFAT have been described that are produced by alternative splicing. In immune related cells, ZFAT is proposed to control apoptosis and cell survival. It also participates in endothelium organization and capillary formation. Studies demonstrated that Zfat regulates the transcription of target genes by binding directly to the TSS proximal region, and that Zfat-target genes play important roles in T-cell homeostasis. Overexpression of ZFAT causes down-regulation of a number of genes involved in the immune response. ZFAT is down-regulated in preeclampsia. Polymorphism in ZFAT is linked with severity in Hashimoto s disease (HD). This rat monoclonal antibody is validated in Western Blotting, Immunoprecipitation, Immunofluorescence, Chromatin Immunoprecipitation. (Ref.:Ishikura, S., et al. (2016). Biochim Biophys Acta. 1859(11):1398-1410; Ishikura, S., et al. (2021). J Biol Chem. 297(4):101213).

GST-tagged, C-terminal recombinant fragment corresponding to 164 amino acids of mouse ZFAT.

Quality Control Testing

Evaluated by Western Blotting in HEK-293 cells transfected with mouse ZFAT.

Western Blotting Analysis: A 1:100,000 dilution from a representative lot detected ZFAT in HEK-293 cells transfected with mouse ZFAT but not in HEK-293 cells transfected with human ZFAT.

Tested Applications

ChIP-seq: A representative lot detected ZFAT in ChIP-seq application (Ishikura, S., et al. (2016). Biochim Biophys Acta. 1859(11):1398-1410).

Immunoprecipitation Analysis: A representative lot Immunoprecipitated ZFAT (Ishikura, S., et al. (2016). Biochim Biophys Acta. 1859(11):1398-1410; Ishikura, S., et al. (2021). J Biol Chem. 297(4):101213).

Western Blotting Analysis: A representative lot detected ZFAT in Western Blotting application (Ishikura, S., et al. (2016). Biochim Biophys Acta. 1859(11):1398-1410; Ishikura, S., et al. (2021). J Biol Chem. 297(4):101213).

Immunofluorescence Analysis: A representative lot detected ZFAT in Immunofluorescence application (Ishikura, S., et al. (2021). J Biol Chem. 297(4):101213).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected ZFAT in Chromatin Immunoprecipitation application (Ishikura, S., et al. (2016). Biochim Biophys Acta. 1859(11):1398-1410; Ishikura, S., et al. (2021). J Biol Chem. 297(4):101213).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone Z4 is a rat monoclonal antibody that specifically detects ZFAT. It targets an epitope within 164 amino acids from the C-terminal region.

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.