Anti-BICC1/Protein Bicaudal C Homolog 1 Antibody, clone 2D9.1

Protein bicaudal C homolog 1 (UniProt Q9H694; also known as Bic-C) is encoded by the BICC1 (also known as CYSRD) gene (Gene ID 80114) in human. Drosophila bicaudal C and its mammalian homologs are RNA-binding proteins involved in symmetric distribution and localized translation of mRNAs of many proteins in a wide range of cells and tissues. RNA binding of Bic-C is mediated by N-terminal KH domains (a.a. 132-199 & 284-348), whereas a C-terminal sterile alpha motif (SAM; a.a. 873-936) self-polymerizes and localizes Bic-C at cytoplasmic foci where silencing of bound mRNAs takes place. Defective polymerization results in decreased Bic-C stability and thereby attenuates inhibition of Dishevelled-2 of Wnt/β-catenin pathway. Loss of BICC1 provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning due to defective silencing of Bic-C target mRNAs, including adenylate cyclase 6 (AC6).

~130 kDa observed. 104.8/105.0 kDa (Human/mouse isoform 1) and 94.29/93.41 kDa (Human/mouse isoform 2) calculated.

Epitope: N-terminal half.

Recombinant fragment corresponding to the N-terminal half of human BICC1/Protein Bicaudal C Homolog 1.

Anti-BICC1 Antibody, clone 2D9.1 is an antibody against Protein Bicaudal C Homolog 1 for use in Western Blotting, Immunoprecipitation.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: 1 µg/mL from a representative lot detected Bic-C/BICC1 in human HK and murine mIMCD-3 kidney cell lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY)
Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY).

Clone 2D9.1 reacts with both spliced isoforms of human and murine BICC1 reported by UniProt (Q9H694 & Q99MQ1).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting of recombinant human BICC1.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1.

Concentration: Please refer to lot specific datasheet.

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