Anti-HMGN2, clone EPR7091, Rabbit Monoclonal Antibody

HMGN2 (High Mobility Group Nucleosomal binding domain 2), also known as HMG17, is an abundant, nonhistone chromosomal protein that binds preferentially to nucleosomal core particles of mammalian chromatin. HMGN2 binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It acts as a chromatin-specific transcriptional coactivator that increases the efficiency of initiation of transcription by RNA polymerase II. HMGN2 also plays a role in chromatin assembly and conformation.

~19 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Synthetic peptide corresponding to human HMGN2.

Immunohistochemistry Analysis: A 1:250-500 dilution from a representative lot detected HMGN2 in human colon tissue.

Produced in collaboration with Epitomics

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This Anti-HMGN2 antibody is validated for use in WB, IH for the detection of HMGN2.

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Evaluated by Western Blotting in HL60, K562, HeLa, SH-SY5Y, and Jurkat cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected HMGN2 in HL60, K562, HeLa, SH-SY5Y, and Jurkat cell lysates.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.