生物来源
rat
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
R3, monoclonal
种属反应性
human
技术
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
Epstein-Barr virus ... Ebna-2(3783761)
一般描述
Epstein-Barr病毒(EBV)与几种人类癌症有关,并能使B淋巴细胞活性永生化。EBV是由一小群病毒蛋白引起的,包括永生化所必需的Epstein-Barr核抗原2(EBNA2)。EBNA2是一种属于疱疹病毒科EBNA2家族的转录激活因子,在激活宿主静息B细胞和诱导B细胞增殖时起至关重要的作用。EBNA2具有一个酸性激活结构域,能够招募参与b细胞永生的各种细胞和病毒。已知EBNA2与细胞表型修饰有关,可上调细胞表面受体(如CD21、MYC和CD23)的表达,以及上调病毒基因(如LMP1、LMP2A、LMP2B和EBNA1-6)的表达。
Gross, H., et al. (2010).Virology.397(2): 299-310.
PMID: 19969318
Kremmer, E., et al. (1995).Virology.208(1): 336-342.
PMID: 11831716
PMID: 19969318
Kremmer, E., et al. (1995).Virology.208(1): 336-342.
PMID: 11831716
观察值~ 75 kDa。虽然计算的分子量为49 kDa,但该蛋白通常在~ 75-80 kDa
免疫原
标记GST的重组蛋白,对应于人EBNA2。
表位:EBNA2 C端
应用
免疫沉淀分析: 一个代表性批次已在一个独立实验室中进行了IP测试。(Gross, H., et al. (2010).Virology.397(2):299-310。)
免疫组织化学分析: 代表性批次已被独立实验室用于IH。(Kremmer, E., et al. (1995).Virology.208(1):336-342。)
免疫组织化学分析: 代表性批次已被独立实验室用于IH。(Kremmer, E., et al. (1995).Virology.208(1):336-342。)
抗-EBNA2抗体,克隆R3是一种用于检测EBNA2的大鼠单克隆抗体,也称Epstein-Barr核抗原2,已在WB、IP & IHC中验证。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
生化/生理作用
经证实可与感染EBV的人细胞发生反应。
外形
形式:纯化
纯化的大鼠单克隆IgG2aκ溶于含0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl和0.05%叠氮化钠的缓冲液中。
蛋白G
制备说明
自收到之日起,在2-8°C条件下可稳定保存1年。
分析说明
对照
B95-8细胞裂解液
B95-8细胞裂解液
通过蛋白质印迹对B95-8细胞裂解液进行了评估。
蛋白质印迹分析:0.5 µg/mL该抗体在10 µg B95-8细胞裂解液中检测到EBNA2。
蛋白质印迹分析:0.5 µg/mL该抗体在10 µg B95-8细胞裂解液中检测到EBNA2。
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Matthew R Harter et al.
PLoS pathogens, 12(2), e1005414-e1005414 (2016-02-06)
Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) plays an important role in driving immortalization of EBV-infected B cells through regulating the expression of many viral and cellular genes. We report a structural study of the tumor suppressor BS69/ZMYND11 C-terminal region
Qiu Peng et al.
Journal of virology, 94(7) (2020-01-17)
Biological macromolecule condensates formed by liquid-liquid phase separation (LLPS) have been discovered in recent years to be prevalent in biology. These condensates are involved in diverse processes, including the regulation of gene expression. LLPS of proteins have been found in
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Fang Lu et al.
Journal of virology, 95(13), e0008821-e0008821 (2021-04-23)
Epstein-Barr virus (EBV) is a human gammaherpesvirus that is causally associated with various lymphomas and carcinomas. Although EBV is not typically associated with multiple myeloma (MM), it can be found in some B-cell lines derived from MM patients. Here, we
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