biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2E12, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PHB(5245)
General description
Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.
30 kDa calculated
Immunogen
Corresponding to human Prohibitin.
Application
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Prohibitin in 1X10E6 Jurkat T cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Prohibitin in MRC5 cells.
Flow Cytometry Analysis: A representative lot detected Prohibitin in Jurkat cells (Thomas Moran, Mount Sinai School of Medicine, New York, NY).
Immunocytochemistry Analysis: A representative lot detected Prohibitin in HeLa cells (Thomas Moran, Mount Sinai School of Medicine, New York, NY).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Prohibitin in MRC5 cells.
Flow Cytometry Analysis: A representative lot detected Prohibitin in Jurkat cells (Thomas Moran, Mount Sinai School of Medicine, New York, NY).
Immunocytochemistry Analysis: A representative lot detected Prohibitin in HeLa cells (Thomas Moran, Mount Sinai School of Medicine, New York, NY).
This Anti-Prohibitin Antibody, clone 2E12 is validated for use in Immunocytochemistry and Flow Cytometry and Immunoprecipitation for the detection of Prohibitin.
Physical form
Format: Purified
Analysis Note
Evaluated by Immunocytochemistry in HEK293 and NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Prohibitin in HEK293 and NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Prohibitin in HEK293 and NIH/3T3 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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