conjugate
unconjugated
antibody form
purified antibody
clone
D2-9A5, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, flow cytometry: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse
General description
34.48 kDa calculated.
Human IgG1-Fc - fused to a recombinant fragment corresponding to 135 amino acids from the ectodomain region of human NKp46.
Application
Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).
Flow Cytometry Analysis: 4 µg/mL from a representative lot detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel).
ELISA Analysis: A representative lot detected NKp46 (CD335) in ELISA applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).
Flow Cytometry Analysis: 4 µg/mL from a representative lot detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel).
ELISA Analysis: A representative lot detected NKp46 (CD335) in ELISA applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).
Biochem/physiol Actions
Clone D2-9A5 recognizes the whole NKp46 and NKp46-D2, but does not recognize NKp46-D1. It targets an epitope with in 135 amino acids from the extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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