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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
CU18, monoclonal
Technique(s):
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
Application:
ELISA, ICC, IHC, WB
Citations:
-
antibody form
purified antibody
clone
CU18, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
species reactivity
mouse
General description
225-250 kDa calculated.
BCA-225 protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Application
ELISA Analysis: A representative lot detected BCA-225 (BRST-1) in ELISA applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
Western Blotting Analysis: A representative lot detected BCA-225 (BRST-1) in Western Blotting applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
Immunocytochemistry Analysis: A representative lot detected BCA-225 (BRST-1) in Immunocytochemistry applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
Western Blotting Analysis: A representative lot detected BCA-225 (BRST-1) in Western Blotting applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
Immunocytochemistry Analysis: A representative lot detected BCA-225 (BRST-1) in Immunocytochemistry applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
Biochem/physiol Actions
Clone CU18 is a mouse monoclonal antibody that detects human breast carcinoma associated glycoprotein BCA-225.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected BCA-225 (BRST-1) in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected BCA-225 (BRST-1) in human breast cancer tissue sections.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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