Anti-SARS-CoV S-Δ3 fragment Antibody, clone 7G12

Spike glycoprotein (UniProt: P59594; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 1489668) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. Clone 7G12 is a non-neutralizing antibody that specifically binds to the S1 subunit of SARS-CoV spike protein. It recognizes the spike protein (S delta 3).

GST-tagged recombinant fragment corresponding to 294 amino acids from the S1 region, extracellular domain of SARS-CoV spike protein.

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV spike protein.

Western Blotting Analysis (WB): A 1:500,000 dilution of this antibody detected recombinant SARS-CoV spike glycoprotein.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV spike glycoprotein in Immunoprecipitation applications (Ng, O-W., et al. (2014). PLoS One. 9(7):e102415).

Western Blotting Analysis: A representative lot detected SARS-CoV spike glycoprotein in Western Blotting applications (Lip, K-M., et al. (2006) J Virol. 80(2):941-50; Ng, O-W., et al. (2014). PLoS One. 9(7):e102415).

ELISA Analysis: A representative lot detected SARS-CoV spike glycoprotein in ELISA applications (Ng, O-W., et al. (2014). PLoS One. 9(7):e102415).

Fluorescence Activated Cell Sorting (FACS): A representative lot detected SARS-CoV spike glycoprotein in Fluorescence Activated Cell Sorting applications (Ng, O-W., et al. (2014). PLoS One. 9(7):e102415).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV S-D3 fragment, clone 7G12, Cat. No. MABF2214, is a mouse monoclonal antibody that detects SARS-CoV spike glycoprotein and is tested for use in ELISA, FACS, Immunoprecipitation, and Western Blotting.

Clone 7G12 is a mouse monoclonal antibody that detects the S-D3 spike protein of SARS-CoV. It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.