Anti-West Nile Virus Antibody, clone E60

West Nile Virus envelope protein.

~47 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Johnson, B., et. al. (2018). Immunity. 48(3):487-499).

Neutralizing Analysis: A representative lot partially neutralized West Nile Virus infection. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).

Clone E60 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DII fusion loop.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting with full-length ectodomain of West nile virus envelope protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected full-length ectodomain of West nile virus envelope protein.

Virus (West Nile Virus).

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