Human herpesvirus-8 (also known as Kaposi′s sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. ORF45 is an immediate-early phosphoprotein that localizes in the cytoplasm and acts as a viral lytic protein. It interacts with KIF3A, a subunit of kinesin-2 motor protein that transports cargoes along microtubules to cell periphery. The N-terminal amino acids 1-115 and C-terminal amino acids 332-407 of ORF45 are reported to interact with KIF3A. Inhibition of KIF3A-ORF45 interaction is shown to reduce HHV-8 maturation and egress. ORF45 also interacts with interferon regulatory factor 7 and inhibits virus-mediated interferon a/b induction. (Ref.: Sathish, N., et al. (2009). PLoS Pathog 5(3): e1000332; Zhu, FX., et al. (2005). J. Virol. 79(2);800-811; Zhu, FX., and Yuan, Y. (2003). J. Virol. 77(7); 4221-4230).

Clone ORF45 is a mouse monoclonal antibody that detects ORF45 protein in HHV-8 (Kaposi′s sarcoma-associated herpesvirus).

Baculovirus-synthesized ORF45 protein.

Quality Control Testing

Evaluated by Western Blotting in TPA-induced BCBL-1 cell lysates.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected HHV-8 (Kaposi′s sarcoma-associated herpesvirus) ORF45 protein in lysates from 12-O-Tetradecanoylphorbol-13-acetate (TPA; 20 ng/mL; 5-7 days)-induced BCBL-1 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected HHV-8 ORF45 in Western Blotting applications (Zhu, F.X., et al. (2005). J Virol. 79(2):800-11; Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332; De Leo, A., et al. (2017). PLoS Pathog. 13(8):e1006596).

Immunoprecipitation Analysis: A representative lot detected HHV-8 ORF45 in Immunoprecipitation applications (Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332).

Immunocytochemistry Analysis: A representative lot detected HHV-8 ORF45 in Immunocytochemistry applications (Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HHV-8 ORF45, clone ORF45, Cat. No. MABF2257, is a mouse monoclonal antibody that detects ORF45 in HHV-8 (Kaposi′s sarcoma-associated herpesvirus) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.