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Merck
CN

MABF2264

Anti-IRGM1 (LRG-47) Antibody, clone 1B2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1B2, monoclonal
Application:
IF, WB
Citations:
-
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conjugate

unconjugated

antibody form

purified antibody

clone

1B2, monoclonal

purified by

using protein G

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunofluorescence: suitable, western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

46.55 kDa calculated.
A linear peptide corresponding to 15 amino acids from the N-terminal region of mouse Immunity-related GTPase family M protein 1 (IRGM1).

Application

Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in Western Blotting applications (Butcher, B.A., et. al. (2005). Infect Immun. 73(6):3278-86; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).

Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in wild-type, but not in Irgm1 KO NIH3T3 cells treated with IFN-gamma (Courtesy of Brian Fee, Ph.D. and Gregory A. Taylor, Ph.D., Duke University School of Medicine, USA).

Immunofluorescence Analysis: A representative lot detected IRGM1 (LRG-47) in Immunofluorescence applications (Zhao, Y.O., et. al. (2010). PLoS One. 5(1):e8648; Henry, S.C., et. al. (2014). PLoS One. 9(4):e95021; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).

Biochem/physiol Actions

Clone 1B2 is a mouse monoclonal antibody that detects the long form of Immunity-related GTPase family M protein 1 (IRGM1). It targets an epitope within 15 amino acids from the N-terminal region.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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