偶联物
unconjugated
抗体形式
purified antibody
克隆
1B2
monoclonal
纯化方式
using protein G
种属反应性
mouse, mouse, mouse
浓度
(Please refer to lot specific datasheet.)
技术
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG1κ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
46.55 kDa calculated.
A linear peptide corresponding to 15 amino acids from the N-terminal region of mouse Immunity-related GTPase family M protein 1 (IRGM1).
应用
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in Western Blotting applications (Butcher, B.A., et. al. (2005). Infect Immun. 73(6):3278-86; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in wild-type, but not in Irgm1 KO NIH3T3 cells treated with IFN-gamma (Courtesy of Brian Fee, Ph.D. and Gregory A. Taylor, Ph.D., Duke University School of Medicine, USA).
Immunofluorescence Analysis: A representative lot detected IRGM1 (LRG-47) in Immunofluorescence applications (Zhao, Y.O., et. al. (2010). PLoS One. 5(1):e8648; Henry, S.C., et. al. (2014). PLoS One. 9(4):e95021; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in wild-type, but not in Irgm1 KO NIH3T3 cells treated with IFN-gamma (Courtesy of Brian Fee, Ph.D. and Gregory A. Taylor, Ph.D., Duke University School of Medicine, USA).
Immunofluorescence Analysis: A representative lot detected IRGM1 (LRG-47) in Immunofluorescence applications (Zhao, Y.O., et. al. (2010). PLoS One. 5(1):e8648; Henry, S.C., et. al. (2014). PLoS One. 9(4):e95021; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
生化/生理作用
Clone 1B2 is a mouse monoclonal antibody that detects the long form of Immunity-related GTPase family M protein 1 (IRGM1). It targets an epitope within 15 amino acids from the N-terminal region.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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