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Merck
CN

MABF2306

Anti-Adeno-associated virus 8 Antibody, clone HL2383

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
HL2383, monoclonal
Species reactivity:
virus, mouse
Application:
DB, ELISA
Citations:
-
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antibody form

purified antibody

clone

HL2383, monoclonal

purified by

using protein G

species reactivity

virus, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable, dot blot: suitable

isotype

IgG3κ

capacity

neutralization

General description

Recombinant Adeno-associated virus serotype 8 capsid protein.

Application

Dot Blot Analysis: A representative lot detected Adeno-associated virus 8 in Dot Blot applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).

Neutralizing Analysis: A representative lot neutralized Adeno-associated virus infection in cells (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).

ELISA Analysis: A representative lot detected Adeno-associated virus 8 in ELISA applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).

Biochem/physiol Actions

Clone HL2383 is a mouse monoclonal antibody that detects AAV8 capsid protein. Also displays cross-reactivity with AAV3B capsid protein.

Physical form

Purified mouse monoclonal antibody IgG3 in PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Dot Blot with AAV8 and AAV9 virus like particles .

Dot Blot Analysis: Various dilutions of AAV8 and AAV9 virus like particles were probed with Adenovirus 8. This antibody specifically detects AAV8, but not AAV9.

Other Notes

Virus (Adenovirus)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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