偶联物
unconjugated
抗体形式
purified antibody
克隆
TX41
monoclonal
纯化方式
using protein G
种属反应性
rat, mouse, mouse
浓度
(Please refer to lot specific datasheet.)
技术
flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG2aκ
适用性
suitable for immunoassay
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
Ba/F3 cells transfected with murine CLM-8 (MAIR-I/CD300a) fused with the Fc portion of human IgG.
~ 30 kDa observed; 35.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
应用
Flow Cytometry Analysis: A representative lot detected CD300a (CLM-8) in Flow Cytometry applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).
Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).
Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
生化/生理作用
Clone TX41 is a rat monoclonal antibody that specifically detects mouse CD300a (CLM-8).
外形
Purified rat monoclonal antibody in PBS without azide.
制备说明
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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