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Merck
CN

MABF2340

Anti-CD300a (CLM-8) Antibody, clone TX41

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
TX41, monoclonal
Application:
FACS, IF, WB
Citations:
-
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conjugate

unconjugated

antibody form

purified antibody

clone

TX41, monoclonal

purified by

using protein G

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, western blot: suitable

isotype

IgG2aκ

suitability

suitable for immunoassay

UniProt accession no.

target post-translational modification

unmodified

species reactivity

rat

General description

Ba/F3 cells transfected with murine CLM-8 (MAIR-I/CD300a) fused with the Fc portion of human IgG.
~ 30 kDa observed; 35.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Flow Cytometry Analysis: A representative lot detected CD300a (CLM-8) in Flow Cytometry applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).

Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).


Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).

Biochem/physiol Actions

Clone TX41 is a rat monoclonal antibody that specifically detects mouse CD300a (CLM-8).

Physical form

Purified rat monoclonal antibody in PBS without azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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