Anti-CD200/OX2 Antibody, clone OX-90

OX-2 membrane glycoprotein (UniProt: O54901; also known as CD200, MRC OX-2 antigen) is encoded by the Cd200 (also known as Mox2) gene (Gene ID: 17470) in mouse. CD200/OX2 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-259), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200/OX2 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).

Mouse CD200 rat-CD4 fusion protein

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse splenocytes.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD200/OX2 in one million BALB/c mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD200/OX2 in flow cytometry application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71; Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Akkaya, M., et al. (2013). PLoS One. 8(5):e63325).

Enzyme Immunoassay (ELISA): A representative lot detected CD200/OX2 in ELISA application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71).

Immunofluorescence Analysis: A representative lot detected CD200/OX2 in immunofluorescence application (Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83).

Blocking of Interfering Antibodies: A representative lot effectively blocked endogenous binding of CD200/OX2 to CD200R (Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83; Akkaya, M., et al. (2013). PLoS One. 8(5):e63325).

Immunohistochemistry Analysis: A representative lot detected CD200/OX2 in immunohistochemistry application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71; Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone OX-90 is a rat monoclonal antibody that detects CD200/OX2. It targets an epitope within the extracellular domain.

Purified rat monoclonal antibody IgG2a in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.