Anti-Ly-49H/KLRA8 Antibody, clone 3D10

Killer cell lectin-like receptor 8 (UniProt: Q60682; also known as Lymphocyte antigen 49h, Ly-49h, T-cell surface glycoprotein Ly-49H) is encoded by the Klra8 (Gene ID: 16639) in murine species. Ly-49H is a disulfide-linked, homodimeric, single-pass type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-44), a transmembrane domain (aa 45-66), and an extracellular domain (aa 67-266). Its C-type lectin domain is localized in amino acids 143-261. Ly-49H is expressed on a subset of natural killer (NK) cells and serves as a receptor for class I HMC. It can also be co-expressed with Ly-49 inhibitory receptors. However, unlike Ly-49 inhibitory receptors, Ly-49H is not detectable on naive splenic CD31 T cells, indicating that Ly-49H is an NK cell-specific activation receptor. It lacks the immunoreceptor tyrosine-based inhibitory motifs (ITIM) in its cytoplasmic domain but associates with DAP12 that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Its interaction with DAP2 leads to NK cell activation. Clone 3D10 specifically binds Ly-49H and does not cross-react with Ly-49A, C, D, and G2. It abolishes Ly-49H-dependent activation of NK cells. Two isoforms of Ly-49H have been described that are produced by alternative splicing. (Ref.: Forbes, CA., et al. (2014). PLoS Pathog. 10(5); e1004161; Smith, HRC., et al. (2000). J. Exp. Med. 191(8); 1341-1354).

C1498 murine lymphoblasts cells transfected with LY-49A/H.

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected Ly49H/KLRA8 in one million C57BL/6 mouse splenocytes.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ly-49H/KLRA8 in Immunoprecipitation applications (Smith, H.R., et al. (2000). J Exp Med. 191(8):1341-54).

Inhibition Assay: A representative lot of this antibody abolished Ly49H-dependent activation of NK cells.(Forbes, C.A., et al. (2014). PLoS Pathog. 10(5):e1004161).

Flow Cytometry Analysis: A representative lot detected Ly-49H/KLRA8 in Flow Cytometry applications (Smith, H.R., et al. (2000). J Exp Med. 191(8):1341-54; Forbes, C.A., et al. (2014). PLoS Pathog. 10(5):e1004161).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3D10 is a mouse monoclonal antibody that detects Killer cell lectin-like receptor 8 (KLRA8/Ly-49H). It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.