Anti-Megakaryocyte-Erythroid Progenitor Antibody, clone BAH-1

Megakaryocyte/erythroid progenitors (MEPs) are bipotent cells that undergo a fate decision to become either megakaryocytes (Mk) or erythroid (E) cells. Proliferation and differentiation of MEP is shown to be regulated by the presence of thrombopoietin. Clone BAH-1 recognizes c-Mpl, the thrombopoietin receptor with high affinity (Kd = 0.61 nM) and exhibits agonistic activity in stimulating megakaryocytopoiesis and production of mature GpIIb/IIIa-expressing megakaryocytes. However, it does not recognize glycoproteins Ib or IIb/IIIa. This clone is useful in distinguishing subsets of hematopoietic stem cells and myeloid progenitors. (Ref.: Sanada, C., et al. (2016). Blood. 128(7); 923-933; Deng, B., et al. (1998). Blood. 92(6); 1981-1988).

Human megakaryocytic CMK cells.

Quality Control Testing

Evaluate by Western Blotting in human megakaryocytic cells (DAMI) lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected an 84 kDa c-Mpl protein that is expressed on megakaryocytic cells (DAMI).

Tested Applications

Agonist Function: A representative lot of this antibody stimulated c-Mpl Thrombopoietin receptor. (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

Immunoprecipitation Analysis: A representative lot immunoprecipitated c-Mpl in human megakaryocytic CMK cells in Immunoprecipitation applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

ELISA Analysis: A representative lot detected Megakaryocyte-Erythroid Progenitors in ELISA applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

Flow Cytometry Analysis: A representative lot detected a protein expressed on Megakaryocyte-Erythroid Progenitors in Flow Cytometry applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

Western Blotting Analysis: A representative lot detected an 84 kDa protein in Megakaryocyte-Erythroid Progenitors in Western Blotting applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Megakaryocyte-Erythroid Progenitor, clone BAH-1, Cat. No. MABF3070, is a mouse monoclonal antibody that detects 84 kDa antigen in DAMI cells and is used in ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blotting.

Clone BAH-1 is a mouse monoclonal antibody that detects an 84 kD protein in human Megakaryocyte/erythroid progenitor cells (DAMI).

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.