Anti-KIR3DL2/CD158k Antibody, clone Q66

Killer cell immunoglobulin-like receptor 3DL2 (UniProt: P43630; also known as CD158 antigen-like family member K, Natural killer-associated transcript 4, NKAT-4, p70 natural killer cell receptor clone CL-5, p70 NK receptor CL-5, CD158k) is encoded by the KIR3DL2 (also known as CD158K, NKAT4) gene (Gene ID: 3812) in human. KIR3DL2 is a single-pass type I membrane glycoprotein, which is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 22-340), a transmembrane domain (aa 341-360), and a cytoplasmic domain (aa 361-455). It is expressed as a disulfide-linked homodimer and each chain contains three Ig-like domains (42-102; 137-202; and 237-300), and a long cytoplasmic tail that contains two ITIM motifs. Its expression is observed in astrocytes, NK cells, CD8+ T cells, and a small proportion of CD4+ T cells. Its expression is upregulated upon activation of NK and T cells. It serves as a receptor on NK cells and T cells for MHC class I molecules and upon binding of peptide-free HLA-F open conformer, it negatively regulates NK and T cell effector functions. Through interaction with HLA-F, it is suggested to protect motor neurons from astrocyte-induced toxicity. KIR3DL2 also binds to CpG-oligodeoxynucleotides (CpG-ODN), and this ligation induces KIR3DL2 down-modulation from the cell surface and translocation to the endosome to deliver the CpG-ODN to TLR9. Engagement of KIR3DL2 by ODN is reported to induce the internalization of the receptor and caspase-dependent apoptosis of malignant T cells. This apoptotic process is correlated to a dephosphorylation of STAT3 that is constitutively phosphorylated and activated in Se´zary cells. KIR3DL2 is considered as an important diagnostic and prognostic marker for the detection of the tumoral T cell burden of Se´zary syndrome patients. (Ref.: Schmitt, C., et al. (2017). Front. Immunol. 8; 1010; Ghazi, B., et al. (2015). J. Clin. Invest. 135(1); 229-237; Dorothee, G., et al. (2003). Oncogene. 22(46); 7192-7198; Pende, D., et al. (1996). J. Exp. Med. 184(2); 505-518).

NK cells clone DP7 (surface phenotype: CD3-CD16+CD56+GL183-EB6-Z27-).

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected KIR3DL2/CD158k in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Western Blotting Analysis: A representative lot detected KIR3DL2/CD158k in Western Blotting application (Pende, D., et al. (1996). J Exp Med. 184(2):505-18).

Flow Cytometry Analysis: A representative lot detected KIR3DL2/CD158k in Flow Cytometry applications (Pende, D., et al. (1996). J Exp Med. 184(2):505-18; Sivori, S., et al. (2002). Proc Natl Acad Sci USA. 99(7):4526-31; Dorothee, G., et al. (2003). Oncogene.;22(46):7192-8; Bahler, D.W., et al. (2008). Cytometry B Clin Cytom. 74(3):156-62; Carrega, P., et al. (20 9). PLoS One.;4(12):e8132; Falco, M., et al. (2010). J Immunol. 185(1):433-41; Ghazi, B., et al. (2015). J Invest Dermatol. 135(1):229-237).

Immunofluorescence Analysis: A representative lot detected KIR3DL2/CD158k in Immunofluorescence applications (Ghazi, B., et al. (2015). J Invest Dermatol.;135(1):229-237).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone Q66 is a mouse monoclonal antibody that detects Killer cell immunoglobulin-like receptor 3DL2 (KIR3DL2). It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgM in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.