Anti-IL-1α Antibody, clone ALF-161

Quality Control Testing

Evaluated by Western Blotting with His-tagged, recombinant mouse IL-1 protein fragment.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment of mouse IL-1 .

Tested Applications

Inhibition Assay: A representative lot inhibited IL-1 bioactivity in Inhibition assays (Fuhlbrigge, R.C., et al. (1988). J Immunol. 141(8):2643-50).

Immunoprecipitation Analysis: A representative lot immunoprecipitated IL-1 in Immunoprecipitation application (Fuhlbrigge, R.C., et al. (1988). J Immunol. 141(8):2643-50).

ELISA Analysis: A representative lot detected IL-1 in ELISA applications (Fuhlbrigge, R.C., et al. (1988). J Immunol. 141(8):2643-50).

Radioimmunoassay: A representative lot detected IL-1 in Radioimmunoassay applications (Fuhlbrigge, R.C., et al. (1988). J Immunol. 141(8):2643-50).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone ALF-161 is an Armenian hamster monoclonal antibody that detects mouse IL-1 . It targets an epitope near the receptor binding site.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Interleukin-1 alpha (UniProt: P01582; also known as IL-1 alpha) is encoded by the Il1a gene (Gene ID: 16175) in mouse. IL-1 is constitutively expressed in several healthy tissues in a steady state and its expression can be increased in response to a number of stimuli. Absolute amounts of IL-1 vary among cell types, but barrier cells, such as endothelial and epithelial cells, express higher amounts at steady state. IL-1 is synthesized with a propeptide (aa 1-112) and is post-translationally modified by phosphorylation at serine 90, myristoylation on lysine 82, and acetylation on lysine 82. After synthesis, pro- IL-1 is localized into the nucleus, cytosol or lysosomal compartment or is displayed on the outer leaflet of the plasma membrane. Its intracellular localization can change in response to specific stimuli. Pro- IL-1 can also be cleaved by calpain to generate IL-1 -N-terminal domain (NTP) and a mature C-terminal IL-1 form. The C-terminal domain is catalytically active and is capable of signaling via IL-1R. Both IL-1 -NTP and pro- IL-1 contains a functional NLS signal and bind HAX1 to allow translocation into the nucleus. In the nucleus, IL-1 -NTP binds transcription regulation factors and activates expression of proinflammatory cytokines and chemokines independently of IL-1R1 signaling. The biological functions of IL-1 also involve stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. (Ref.: Malik, A., and Kanneganti, TD. (2018). Immunol. Rev. 281(1); 124-137; Di Paolo, NC., and Shayakhmetov, DM (2016). Nat. Commun. 17(8); 906-913).

Full length murine IL-1 .

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Purified armenian hamster monoclonal antibody IgG in PBS without azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.