Anti-BAFF-R/CD268 Antibody, clone 11C1

Tumor necrosis factor receptor superfamily member 13C (UniProt: Q96RJ3; also known as CD268, B-cell-activating factor receptor, BAFF receptor (BAFF-R),BLyS receptor 3) is encoded by the TNFRSF13C (also known as BAFFR, BR3) gene (Gene ID: 115650) in human. BAFF-R/CD268, is a single pass, type III membrane protein in the tumor necrosis factor receptor (TNFR) superfamily. It features an extracellular domain (1-78) with three cysteine-rich motifs, a transmembrane domain (79-99), and a short cytoplasmic tail (100-184). CD268 is crucial for B-cell survival, differentiation, and proliferation. It mediates signaling pathways, including NF- B and MAPK, promoting cell survival and antibody production upon ligand binding. BAFF has been shown to have profound effect on T cell costimulation, through BAFF-R/CD268 as well. CD268 is implicated in diseases like multiple myeloma, B-cell lymphomas and autoimmune diseases. Its overexpression supports malignant plasma cell survival, making it a target for therapeutic interventions in these conditions. (Ref.: Ng, L.G., et al. (2004). J Immunol. 173(2):807-17.)

L1.2 cells transfected with human BAFF-R.

Quality Control Testing

Evaluated by Immunohistochemistry in human tonsil tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CD268/BAFF-R in human tonsil tissue sections.

Tested Applications

Immunohistochemistry Analysis: A representative lot detected BAFF-R/CD268 in Immunohistochemistry application (Ng, L.G., et al. (2004). J Immunol. 173(2):807-17).

Flow Cytometry Analysis: A representative lot detected BAFF-R/CD268 in Flow Cytometry application (Ng, L.G., et al. (2004). J Immunol. 173(2):807-17).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 11C1 is a mouse monoclonal antibody that detects BAFF-R/CD268. It targets an epitope within the human CD268 protein.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.