biological source
rat
conjugate
ALEXA FLUOR™ 647
antibody product type
primary antibodies
clone
53-6.7, monoclonal
species reactivity
mouse
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
Quality Level
General description
T-cell surface glycoprotein CD8 alpha chain, also known as T-cell surface glycoprotein Lyt-2 or CD8a or CD8a, is a member of the immunoglobulin superfamily acting as a co-receptor with MHC class I-restricted T cell receptors, and through its association with protein tyrosine kinase p56Ick, modulates antigen recognition and T cell activation as well as thymic development. CD8a is expressed on the majority of thymocytes, a subset of mature T lymphocytes, and NK cells (almost exclusively CD8a homodimers).
27 kDa calculated
Application
Anti-CD8a (mouse) Antibody, AlexaFluor647, clone 53-6.7 is a highly specific rat monoclonal antibody, that targets CD8 & has been tested in Flow Cytometry, IHC & IP.
Analysis Note
The antibody was purified by affinity chromatography, and conjugated with AlexaFluor488 under optimal conditions. The solution is free of unconjugated AlexaFluor488 and unconjugated antibody. Alexa Fluor is a registered trademark of Molecular Probes, Inc. Alexa Fluor dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD8a in one million C57BL/6 mouse splenocytes.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD8a in one million C57BL/6 mouse splenocytes.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
10-13 - German Storage Class 10 to 13
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