Anti-APOBEC3A Antibody, clone 01D05

DNA dC->dU-editing enzyme APOBEC-3A (UniProt: P31941; EC:3.5.4.38; also known as Phorbolin-1, A3A) is encoded by the APOBEC3A gene (Gene ID: 200315) in human. APOBEC3A is a member of the APOBEC family of cytidine deaminases, that catalyze the deamination of cytidine to uridine in single-stranded DNA. APOBEC3A is characterized by a single cytidine deaminase domain. APOBEC3A plays a role in innate immunity by restricting the replication of various viruses and retroelements, by introducing mutations into single stranded DNA and RNA. It can also induce mutations in host DNA. APOBEC3A is implicated in cancer development due to its ability to introduce mutations in genomic DNA, contributing to mutational signatures observed in several cancer types, including breast cancer. Its mutagenic activity is associated with genomic instability, which can lead to tumorigenesis. (Ref: Dananberg, A. and Maciejowski, J., (2024), Methods Cell Biol. 182; 313 327)

KLH-conjugated linear peptide corresponding to 43 amino acids from the N-terminal of human APOBEC3A.

Quality Control Testing

Evaluated by Western Blotting in BT-474 cell lysate.

Western Blotting Analysis A 1:500 dilution of this antibody detected APOBEC3A in BT-474 cell lysate.

Quality Control Testing

Evaluated by Western Blotting in BT-474 cell lysate.

Western Blotting Analysis A 1:500 dilution of this antibody detected APOBEC3A in BT-474 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected APOBEC3A in BT-474 cell lysate.

ELISA Analysis: A representative lot detected APOBEC3A in ELISA application (Petljak, M., et al. (2022). Nature. 607(7920):799-807; Dananberg, A., et al. (2024). Methods Cell Biol. 182:313-327).

Western Blotting Analysis: A representative lot detected APOBEC3A in Western Blotting application (Petljak, M., et al. (2022). Nature. 607(7920):799-807; Dananberg, A., et al. (2024). Methods Cell Biol. 182:313-327).

Immunofluorescence Analysis: A representative lot detected APOBEC3A in Immunofluorescence application (Dananberg, A., et al. (2024). Methods Cell Biol. 182:313-327).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 01D05 is a mouse recombinant monoclonal antibody that detects APOBEC3A. It targets an epitope within 43 amino acids from the N-terminal.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.