biological source
mouse
conjugate
APC-Cy7
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
A20, monoclonal
species reactivity
mouse
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Ptprc(19264)
General description
The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Application
Anti-CD45.1 (mouse), APC-Cy7, clone A20 | MABF433 is an antibody against CD45.1 for use in Immunoprecipitation, Immunohistochemistry, Flow Cytometry.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.
Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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