Anti-EBI3 Antibody/IL-27B, clone V1.4H5.29

EBI3/IL27B is the beta subunit of the functional cytokine IL-27. The beta subunit is required for activity. IL-27 is a critical cytokine of mammalian innate immune response. IL-27 has both pro and anti-inflammatory activities. IL-27 regulates T-helper cell development and differentiation into TH1, TH2 and TH17 effector cells. IL-27 also is important in isotype switching for B-cells, and it drives rapid clonal expansion of native but not memory CD4 cells. IL-27 is also important for its anti-tumor and anti-angiogenic activity on tumor and cancer cells. The beta subunit EBI3/IL27B is also a component of IL-35 which is a heterodimer of IL-12alpha and IL-27B/EBI3. IL-35 plays a role in immune suppression. IL-27 is a secreted cytokine, thus IL-27beta subunit is expressed intracellularly associated with the endoplasmic reticulum and membrane vesicles and is detected extracellularly as part of the mature cytokine as well.

~23 kDa observed. Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to mouse EBI3/IL-27B.

Anti-EBI3 Antibody/IL-27B, clone V1.4H5.29 | MABF835 is an antibody against EBI3 Antibody/IL-27B for use in Western Blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Western Blotting Analysis: A representative lot detected EBI3/IL-27B in T reg cells from mouse tissue lysate (Collison, L.W., et al. (2010). National Institute of Health. 11(12):1093-1101).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing PBS without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in recombinant protein mouse EBI3.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected EBI3/IL-27B in 0.1 µg of recombinant protein mouse EBI3.

Concentration: Please refer to lot specific datasheet.

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