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Merck
CN

MABF839

Anti-Ly-6G Antibody, clone 1A8 (Azide free)

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1A8, monoclonal
Application:
FACS, IP, activity assay
Citations:
-
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conjugate

unconjugated

antibody form

purified antibody

clone

1A8, monoclonal

purified by

using protein G

species reactivity

mouse, rat

concentration

(Please refer to lot specific datasheet.)

technique(s)

activity assay: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable

isotype

IgG2aκ

UniProt accession no.

target post-translational modification

unmodified

Quality Level

General description

12 kDa calculated
Propeitary whole cells expressing to mouse Ly-6G.

Application

Flow Cytometry Analysis: 0.5 µg of this antibody from a representative lot detected Ly-6G in 1X10E6 mouse bone marrow cells.
Flow Cytometry Analysis: A representative lot detected Ly-6G in transfected EL4 cells and bone marrow cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
Activity Assay Analysis: A representative lot detected Ly-6G in transmission of S. pneumoniae from index after injection with this clone (Short, K.R., et al. (2013). mBio. 3(5):e00255-12).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ly-6G in transfected EL4 cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).

Physical form

Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Flow Cytometry in mouse splenocyte.

Flow Cytometry Analysis: 0.5 µg of this antibody detected Ly-6G in mouse splenocyte.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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