biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6C3.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... LIMK1(3984)
General description
LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.
~72 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human Limk1.
Application
Detect Limk1 using this Anti-Limk1 Antibody, clone 6C3.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Limk1 in human thalamus and human cerebral cortex tissue.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in human cortex tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Limk1 in 10 µg of human cortex tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Limk1 in 10 µg of human cortex tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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