Anti-DGCR8 Antibody, clone 11C10.1

Microprocessor complex subunit DGCR8 (UniProt Q8WYQ5; also known as DiGeorge syndrome critical region 8, Pasha) is encoded by the DGCR8 (also known as DGCRK6, LP4941) gene (ORF C22orf12; Gene ID 54487) in human. The DGCR8 gene encodes a double-stranded RNA-binding protein that functions as a non-catalytic subunit of the microprocessor complex which mediates the biogenesis of microRNAs (miRNA) from the primary microRNA (pri-microRNA) transcript. miRNAs are initially transcribed as pri-microRNA with a local stem-loop that is cleaved by the microprocessor complex to produce the precursor microRNA (pre-miRNA). The microprocessor complex is comprised of the RNase Drosha and the RNA-binding protein DGCR8. DGCR8 is reported to be phosphorylated by Erk and Abl, an event that promotes DGCR8 stability and stimulates Drosha recruitment and subsequent substrate pri-microRNAs processing.

~120 kDa observed. 86.05 kDa (isoform 1) and 82.75 kDa (isoform 3). The detected band size is consistent with the reported apparent molecular weight (Ha, M., and Kim, V.N. (2014). Nat. Rev. Mol. Cell Biol. 15(8):509-524).

Epitope: Region between WW and DRBM 1 domain.

GST-tagged recombinant fragment from the region between the WW and DRBM 1 domain of human DGCR8.

Anti-DGCR8 Antibody, clone 11C10.1 is an antibody against DGCR8 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
RNA Metabolism & Binding Proteins

Expected to react with spliced isoforms 1 and 3, but not isoform 2 of human DGCR8 reported by UniProt (Q8WYQ5).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DGCR8 in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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