conjugate
unconjugated
antibody form
purified antibody
clone
20F8, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse, rat
General description
GST-tagged full-length mouse LIM domain transcription factor LMO4.
~18 kDa observed; 17.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated LMO4 in Immunoprecipitation applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected LMO4 in Immunohistochemistry applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Murphy, N.C., et. al. (2008). Br J Cancer. 98(3):537-41; Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Western Blotting Analysis: A representative lot detected LMO4 in Western Blotting applications (Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected LMO4 in Immunohistochemistry applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Murphy, N.C., et. al. (2008). Br J Cancer. 98(3):537-41; Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Western Blotting Analysis: A representative lot detected LMO4 in Western Blotting applications (Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Biochem/physiol Actions
Clone 20F8 is a rat monoclonal antibody that detects LIM domain transcription factor LMO4 in murine species.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LMO4 in MCF7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LMO4 in MCF7 cell lysate.
Other Notes
Mouse. Predicted to react with Bovine and Human.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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