偶联物
unconjugated
抗体形式
purified antibody
克隆
20F8
monoclonal
纯化方式
using protein G
种属反应性
mouse, human, bovine, human, rat, bovine
浓度
(Please refer to lot specific datasheet.)
技术
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG2aκ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
GST-tagged full-length mouse LIM domain transcription factor LMO4.
~18 kDa observed; 17.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
应用
Immunoprecipitation Analysis: A representative lot immunoprecipitated LMO4 in Immunoprecipitation applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected LMO4 in Immunohistochemistry applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Murphy, N.C., et. al. (2008). Br J Cancer. 98(3):537-41; Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Western Blotting Analysis: A representative lot detected LMO4 in Western Blotting applications (Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected LMO4 in Immunohistochemistry applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Murphy, N.C., et. al. (2008). Br J Cancer. 98(3):537-41; Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
Western Blotting Analysis: A representative lot detected LMO4 in Western Blotting applications (Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
生化/生理作用
Clone 20F8 is a rat monoclonal antibody that detects LIM domain transcription factor LMO4 in murine species.
外形
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LMO4 in MCF7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LMO4 in MCF7 cell lysate.
其他说明
Mouse. Predicted to react with Bovine and Human.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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