偶联物
unconjugated
抗体形式
purified antibody
克隆
11G2
monoclonal
纯化方式
using protein G
种属反应性
human, mouse, human
浓度
(Please refer to lot specific datasheet.)
技术
electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
84.14 kDa calculated.
Mixture of HEL human erythroleukemia cells and Jurkat cells.
应用
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM10 in Immunoprecipitation applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Western Blotting Analysis: A representative lot detected ADAM10 in Western Blotting applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Immunocytochemistry Analysis: A representative lot detected ADAM10 in Immunocytochemistry applications (Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Electron Microscopy Analysis: A representative lot complexed with ADAM10 (ADAM10-11G2 Fab) was visualized by negative stain electron microscopy and single-particle averaging. (Seegar, T.C.M., et. al. (2017). Cell. 171(7);1638-1648).
Flow Cytometry Analysis: A representative lot detected ADAM10 in Flow Cytometry applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Western Blotting Analysis: A representative lot detected ADAM10 in Western Blotting applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Immunocytochemistry Analysis: A representative lot detected ADAM10 in Immunocytochemistry applications (Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Electron Microscopy Analysis: A representative lot complexed with ADAM10 (ADAM10-11G2 Fab) was visualized by negative stain electron microscopy and single-particle averaging. (Seegar, T.C.M., et. al. (2017). Cell. 171(7);1638-1648).
Flow Cytometry Analysis: A representative lot detected ADAM10 in Flow Cytometry applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
生化/生理作用
Clone 11G2 is a mouse monoclonal antibody that specifically detects human Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10).
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Evaluated by Flow Cytometry in Jurkat cells.
Flow Cytometry Analysis: 1 µg of this antibody detected ADAM10 in one million Jurkat cells.
Flow Cytometry Analysis: 1 µg of this antibody detected ADAM10 in one million Jurkat cells.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
法规信息
新产品
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持