MABN2317
Anti-ADAM10 Antibody, clone 11G2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
11G2, monoclonal
Application:
EM, FACS, ICC, IP, WB
Citations:
-
conjugate
unconjugated
antibody form
purified antibody
clone
11G2, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
electron microscopy: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse
General description
84.14 kDa calculated.
Mixture of HEL human erythroleukemia cells and Jurkat cells.
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM10 in Immunoprecipitation applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Western Blotting Analysis: A representative lot detected ADAM10 in Western Blotting applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Immunocytochemistry Analysis: A representative lot detected ADAM10 in Immunocytochemistry applications (Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Electron Microscopy Analysis: A representative lot complexed with ADAM10 (ADAM10-11G2 Fab) was visualized by negative stain electron microscopy and single-particle averaging. (Seegar, T.C.M., et. al. (2017). Cell. 171(7);1638-1648).
Flow Cytometry Analysis: A representative lot detected ADAM10 in Flow Cytometry applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Western Blotting Analysis: A representative lot detected ADAM10 in Western Blotting applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Immunocytochemistry Analysis: A representative lot detected ADAM10 in Immunocytochemistry applications (Jouannet, S., et. al. (2016). Cell Mol Life Sci. 73(9):1895-915; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Electron Microscopy Analysis: A representative lot complexed with ADAM10 (ADAM10-11G2 Fab) was visualized by negative stain electron microscopy and single-particle averaging. (Seegar, T.C.M., et. al. (2017). Cell. 171(7);1638-1648).
Flow Cytometry Analysis: A representative lot detected ADAM10 in Flow Cytometry applications (Saint_Pol, J., et. al. (2017). J Biol Chem. 292(23):9551-9566; Stoeck, A., et. al. (2006). Biochem J. 393(Pt 3):609-18; Arduise, C., et. al. (2008). J Immunol. 181(10):7002-13).
Biochem/physiol Actions
Clone 11G2 is a mouse monoclonal antibody that specifically detects human Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Flow Cytometry in Jurkat cells.
Flow Cytometry Analysis: 1 µg of this antibody detected ADAM10 in one million Jurkat cells.
Flow Cytometry Analysis: 1 µg of this antibody detected ADAM10 in one million Jurkat cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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