Anti-mutant HTT Antibody, clone PHP3

347.60 kDa calculated.

GST-tagged recombinant exon1 of wild-type human huntingtin HTTx1 (20Q) protein.

Immunohistochemistry Analysis: A representative lot detected mutant HTT in Immunohistochemistry applications (Ko, J., et. al. (2018). Hum. Mol. Genet. 27(13): 2330-2343).

Inhibits Activity/Function Analysis: A representative lot partially blocked seeding and subsequent fibril assembly of mHTTx1 in cell culture models of HD. (Ko, J., et. al. (2018). Hum. Mol. Genet. 27(13): 2330-2343).

Dot Blot Analysis: A representative lot detected mutant HTT in Dot Blotting applications (Ko, J., et. al. (2018). Hum. Mol. Genet. 27(13): 2330-2343).

Electron Microscopy Analysis: A representative lot detected mutant HTT in Electron Microscopy applications (Ko, J., et. al. (2018). Hum. Mol. Genet. 27(13): 2330-2343).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mutant HTT in brain tissue lysates of 6 and 9 month-old WT or Q140 KI HD mice (Courtesy of Ali Khoshnan, Ph.D., California Institute of Technology USA).

Western Blotting Analysis: A representative lot detected mutant HTT in Western Blotting applications (Ko, J., et. al. (2018). Hum. Mol. Genet. 27(13): 2330-2343).

Clone PHP3 is a mouse monoclonal antibody that detects Huntington protein. It targets an epitope within polyQ-polyP junction (QQQQPPPP). It recognizes some early oligomers.

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Immunohistochemistry (Paraffin) in Human Huntington′s diseased brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected mutant HTT in Human Huntington′s diseased brain tissue sections.

Human, Mouse.

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