Anti-Neurofilament associated-Mauthner cells Antibody, clone 3A10

Mauthner cells, large, identifiable neurons in the vertebrate brain, play a crucial role in the escape response that can be triggered by abrupt or threatening events. These cells are known for their long axons that project caudally in the spinal cord in the vicinity of motoneuron cell bodies and allow for rapid transmission of signals. These cells are characterized by their prominent expression of neurofilaments that provides structural support to their long axons and are often used as a marker to identify these neurons. Neurofilaments in Mauthner cell are important for maintaining the structural integrity of these large neurons and facilitate the transport of organelles and proteins along the axon. These cells exhibit mixed type of synaptic contacts with electrical and chemical synapses and large myelinated club endings. They have been used for a wide range of studies, including simultaneous recordings from the presynaptic and postsynaptic regions. When these cells are activated, the extracellular currents associated with their action potential are reported to produce a negative field potential that can be as large as 20-40 mV close to the axon hillock. Clone 3A10 is suitable for use in multiple applications, including Western Blotting, immunohistochemistry, immunocytochemistry, and immunofluorescence and displays reactivity in multiple species. (Ref.: Hecker, A., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(6); 3254-3260; Pineda, RH., et al. (2006). Development. 133(19); 3827-3836; Korn, H., and Faber, DS. (2005). Neuron. 47(1); 13-28).

Cyclophosphamide treated chicken Ventral spinal cord tissue.

Quality Control Testing

Evaluated by Immunohistochemistry in mouse embryo brain tissue sections.

Immunohistochemistry (Paraffin) Analysis IHC): A 1:50 dilution from a representative lot detected Neurofilament associated-Mauthner cells in E16.5 mouse embryo brain tissue sections.

Tested Applications

Immunohistochemistry Analysis: A representative lot detected NF associated-Mauthner cells in Immunohistochemistry application (Wang, G., et al. (2008). Dev Biol. 321(1):216-26; Manitt, C., et al. (2009). J Neurosci. 29(36):11065-77; Bonner, J., et al. (2012). Neural Dev. 6:7:18; Prats, E., et al. (2017). Sci Rep. 7(1):13952; Panlilio, J.M., et al. (2023). Sci Rep. 13(1):2587).

Western Blotting Analysis: A representative lot detected NF associated-Mauthner cells in Western Blotting application (Liang, C., et al. (2017). Invest Ophthalmol Vis Sci. 58(12):5485-5496).

Immunocytochemistry Analysis: A representative lot detected NF associated-Mauthner cells in Immunocytochemistry application (Pineda, R.H., et al. (2006). Development. 133(19):3827-36; Lance-Jones, C., et al. (2012). Dev Neurobiol. 72(2):167-85; Shim, S., et al. (2013). Mol Brain. 1:6:51).

Immunofluorescence Analysis: A representative lot detected NF associated-Mauthner cells in Immunofluorescence application (Fidler, P.S., et al. (1999). J Neurosci. 19(20):8778-88; Pineda, R.H., et al. (2006). Development. 133(19):3827-36; Wang, G., et al. (2008). Dev Biol. 321(1):216-26; Yoon, B.C., et al. (2012). Cell. 148(4):752-64; Bonner, J., et al. (2012). Neural Dev. 6:7:18; Shim, S., et al. (2013). Mol Brain. 1:6:51).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3A10 is a mouse monoclonal antibody that specifically detects Neurofilament associated antigen. The antibody labels a subset of hindbrain spinal cord projecting neurons such as Mauthner neurons.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.