Anti-Guanylate cyclase 1 Antibody, clone IS4

Retinal guanylyl cyclase 1 (UniProt: P55203; also known as EC:4.6.1.2, RETGC-1, Guanylate cyclase 2D, retinal, Guanylate cyclase E (GC-E), Rod outer segment membrane guanylate cyclase, ROS-GC) is encoded by the GUCY2D (also known as GUC2D) gene (Gene ID: 282245) in bovine. Guanylate cyclase 1 is a membrane-bound retinal guanylyl cyclase expressed predominantly in the cone and rod photoreceptors in the cone outer segment. Guanylate cyclase 1 and the related Guanylate cyclase 2 mediate the synthesis of cyclic 3 , 5 -guanosine monophosphate (cGMP) from guanosine triphosphate in mammalian photoreceptor cells, where Guanylate cyclase 1 and its associated activator proteins are responsible for the Ca2+-sensitive restoration of cGMP levels after light activation of the photo transduction cascade. In bovine, Guanylate cyclase 1 is produced with a signal peptide (aa 1-56) sequence, the removal of which yields the mature enzyme with an extracellular domain (aa 57-467), followed by a transmembrane segment (aa 468-492) and a cytoplasmic tail (aa 493-1110) that contains a protein kinase homology domain (aa 493-818) and the guanylate cyclase catalytic domain (aa 885-1015). Heterozygous mutations in the GUCY2D gene have been shown to cause autosomal dominant cone and cone-rod dystrophies (adCODs and adCORDs, respectively), while homozygous or compound heterozygous mutations cause autosomal recessively inherited Leber Congenital Amaurosis (LCA), the most severe form of inherited retinopathy, resulting in total blindness or greatly impaired vision at birth or in early infancy. (Ref.:Haire, S.E., et al. (2006). Invest Ophthalmol Vis Sci. 47(9):3745-53; Ying, G., et al. (2018). J Biol Chem. 293(45):17546-17558).

Linear peptide corresponding to 15 amino acids from the C-terminal region of bovine Retinal guanylyl cyclase 1.

Clone IS4 is a mouse monoclonal antibody that detects an epitope within 15 amino acids from the C-terminal region of bovine Retinal guanylyl cyclase 1.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

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