Anti-Synaptotagmin-12 Antibody, clone N277/7

Synaptotagmin-12 is also called Synaptotagmin XII (SytXII).Synaptotagmin-12 is a member of the synaptotagmin family of transmembrane transporters. Synaptotagmin-12 is thought to be involved in Ca2+-dependent exocytosis of secretory vesicles. Synaptotagmin-12 may also act as Ca2+ sensors as part of the vesicular trafficking and exocytosis process.

~47 kDa observed.

Recombinant protein corresponding to mouse Synaptotagmin-12.

Detect Synaptotagmin using this mouse monoclonal antibody, Anti-Synaptotagmin-12 Antibody, clone N277/7 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Synpatotagmin-12 in mouse brain and rat cerebellum tissues.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Synaptotagmin-12 in 10 µg of human brain tissue lysate.

Western Blotting Analysis: A representative lot detected Synaptotagmin-12 in COS cell lysates transfected with GFP-tagged Synaptotagmin-12, and not in COS cell lysates transiently transfected with other GFP-tagged Synaptotagmins or with untagged Kv2.1 plasmid (Prof. J. Trimmer, University of California, Davis.). Note that GFP tagged proteins run higher than the expected molecular weight of the target protein.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This antibody demonstrates specificity for Synaptotagmin-12, and does not cross react with 11 other Synaptotagmins.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain tissue lysate

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Synpatotagmin-12 in 10 µg of mouse brain tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.