MABN529
Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody
clone 6F12.2, from mouse
别名:
Neuronal acetylcholine receptor subunit alpha-7, acetylcholine receptor alpha-7, Neuronal acetylcholine receptor subunit alpha7, acetylcholine receptor alpha7, nAChRa7, nAChRa-7
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F12.2, monoclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CHRNA7(1139)
General description
The protein named Neuronal acetylcholine receptor subunit alpha-7, or neuronal nicotinic acetylcholine receptor alpha7 subunit (nAChRa7) is the alpha subunit of a multi-protein, post-synaptic membrane bound ion-channel receptor complex expressed in acetylcholine responsive neurons in the brain and some immune cells. The nAChRa7 receptor belongs to the family of ligand gated ion channels. In the brain, the nAChRa7 receptor is expressed in key brain regions and it is involved in essential cognitive functions such as memory, thinking, comprehension, learning capacity, calculation, orientation, language, and judgment. The a7 subunit has also been identified as a significant mediator of nicotine interactions within the stress axis and human disease. nAChRa7 also has neuroprotective effects via multiple pathways making it a target for treatment of a number of brain disorders including Alzheimer’s disease. Finally, nAChRa7 is key to the cholinergic anti-inflammatory pathway in mediating inflammatory responses by immune cells which can aid in chronic inflammatory diseases like Atherosclerosis.
~60 kDa observed. Uncharacterized bands may be observed in some lysates.
Immunogen
GST-tagged recombinant protein corresponding to the cytoplasmic domain of Human CHRNA7.
Application
This Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Neuronal acetylcholine receptor α-7.
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Neuronal acetylcholine receptor α-7 in 10 µg of Mouse Hypothalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Neuronal acetylcholine receptor α-7 in human cerebellum, cerebral cortex, and thalamic tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Neuronal acetylcholine receptor α-7 in human cerebellum, cerebral cortex, and thalamic tissue.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in mouse cerebellum tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neuronal acetylcholine receptor α-7 in 10 µg of mouse cerebellum tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neuronal acetylcholine receptor α-7 in 10 µg of mouse cerebellum tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Brijesh K Garg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 65(9), 499-512 (2017-08-02)
Alpha7 nicotinic acetylcholine receptors (α7 nAChRs) are important drug targets in neurological disorders and inflammation, making their detection and localization by validated antibodies highly desirable. However, tests in knockout animals raised questions about specificity of antibodies to mouse α7 nAChRs.
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