Inositol polyphosphate 5-phosphatase OCRL-1 (ORCL) is also called Lowe oculocerebrorenal syndrome protein. ORCL is a phosphotase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. OCRL defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).

~104 kDa observed. Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to human OCRL/INPP5b.

Detect Inositol polyphosphate 5-phosphatase OCRL-1 using this mouse monoclonal antibody, Anti-OCRL/INPP5b Antibody, clone N166A/26 validated for use in western blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected OCRL/INPP5b in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected OCRL/INPP5b in COS cell lysates transiently transfected with GFP-tagged OCRL or RFP-tagged INPP5b, and demonstrated a loss of signal in COS cell lysates transiently transfected with untagged Kv2.1 plasmids (Prof. J. Trimmer, University of California, Davis.).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OCRL/INPP5b in human cerebellum and human cardiac myocytes.

Cross-reacts with OCRL/INPP5b (Prof. J. Trimmer, Univerity of California, Davis).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain tissue lysate

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected OCRL/INPP5b in 10 µg of human brain tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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