Anti-p70S6 Kinase Antibody, clone 10H11.1

Ribosomal protein S6 kinase beta-1 (UniProt: P23443; also known as EC 2.7.11.1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA) is encoded by the RPS6KB1 (also known as STK14A) gene (Gene ID: 6198) in human. P70S6K1 is a serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth, and cell cycle progression. It regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Upon mitogenic stimulation, phosphorylation by mTORC1 leads to its activation following dissociation from the EIF3 complex. The active p70S6K1 then phosphorylates and activates several substrates in the pre-initiation complex. It is also shown to play a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, which results in the inhibition of mTORC2 and Akt1 signaling. The protein kinase domain of p70S6K1 is localized to amino acids 910352 and the autoinhibitory domain is localized to amino acids 424-525. The autoinhibitory domain blocks phosphorylation within the AGC-kinase C-terminal domain and the activation loop. It also contains a TOR signaling (TOS) motif (aa 28-32) that is essential for its activation by mTORC1. Five isoforms of p70S6K1 have been described that are produced by alternative splicing and alternative initiation.

~70 kDa observed; 59.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

GST/His-tagged recombinant fragment corresponding to the first 93 amino acids from the N-terminal region of human Ribosomal protein S6 kinase beta-1.

Anti-p70S6 Kinase, clone 10H11.1, Cat. No. MABS1687, is a mouse monoclonal antibody that detects Ribosomal protein S6 kinase beta-1 and has ben tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected p70S6 Kinase in human kidney and human liver tissue sections.

Research Category
Signaling

Clone 10H11.1 is a mouse monoclonal antibody that detects human Ribosomal protein S6 kinase beta-1. It targets an epitope within the first 93 amino acids from the N-terminal region.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected p70S6 Kinase in HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

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