Anti-FNIP1 Antibody, clone 1C10.2

Folliculin-interacting protein 1 (UniProt Q8TF40; FNIP1 protein) is encoded by the FNIP1 (also known as KIAA1961) gene (Gene ID 96459) in human. FNIP1 and 2 proteins are homologous partners of folliculin (FLCN) and are required for FLCN localization to lysosomes during amino acid starvation. FNIP1 protein is an evolutionary conserved 130 kDa protein that is shown to interact with AMPK and can serve as a substrate for activated AMPK. Blocking of AMPK activity is reported to reduce FNIP protein expression in HEK293 cells. FNIP1 is also involved in autophagic process where it interacts with GABARAP of the ATG8 family and knockdown of FNIP1 is shown to enhance autophagosome formation. Strong expression of FNIP1 protein is reported in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels have been reported in renal cell carcinoma (RCC). Loss of FNIP1 protein is also shown to mitigate muscle damage in a murine model of Duchenne muscular dystrophy. Ref.: Baba, M., et al. (2006). Proc. Natl. Acad. Sci. USA 103, 15552 15557. Hasumi, H., et al. (2015). Proc. Natl. Acad. Sci. USA 112, 1624-1631.

~130 kDa observed. 130.6/127.4 kDa (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human FNIP1.

Research Category
Signaling

Detect FNIP1 using this mouse monoclonal Anti-FNIP1 Antibody, clone 1C10.2, Cat. No. MABS1717, validated for use in Western Blotting.

Epitope region is present in spliced isoforms 1 and 3, but not 2, of human FNIP1 reported by UniProt (Q8TF40).

Format: Purified

Protein G purified.

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FNIP1 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

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