Anti-TMEM64 Antibody, clone 7A2.1

Transmembrane protein 64 (UniProt Q6YI46) is encoded by the TMEM64 gene (Gene ID 169200) in human. TMEM64 is a 6-transmembrane protein that plays a role in modulating Wnt/ -catenin signaling-mediated osteoblast and adipocyte differentiation. TMEM64 interacts and positively regulates sarcoplasmic endoplasmic reticulum Ca(2+) ATPase 2 (SERCA2) activity, which is important for RANKL-induced Ca(2+) oscillation, Ca(2+)/calmodulin-dependent protein kinases (CaMK) IV activity and mitochondrial ROS formation needed for for osteoclast formation. Mice with Tmem64 deficiency are characterized by a high-bone-mass phenotype, displaying higher levels of ALP, osteocalcin (Ocn), and type 1 collagen (Col 1a) transcripts than in wild-type mice. Tmem64-knockout in mice is reported to markedly enhance osteoblast differentiation and inhibit adipocyte differentiation from bone marrow-derived stromal cells (BMSCs), resulting in a preferential switch of mesenchymal progenitors favoring the osteogenic over the adipogenic lineage.

~45 kDa observed. 39.67/12.95/14.25/33.97 kDa (human isoform 1/2/3/4) and 39.53 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human and rat TMEM64.

Detect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of SK-MEL-28 cell lysate.

The immunogen sequence is 100% conserved between human and rat species and present in all four human spliced isoforms reported by UniProt (Q6YI46 & D3ZV19). Murine cross-reactivity is highly likely, but has not been confirmed.

Protein G purified.

Format: Purified

Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of A431 cell lysate.

Concentration: Please refer to lot specific datasheet.

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