Anti-TMEM64 Antibody, clone 7A2.1

Transmembrane protein 64 (UniProt Q6YI46) is encoded by the TMEM64 gene (Gene ID 169200) in human. TMEM64 is a 6-transmembrane protein that plays a role in modulating Wnt/ -catenin signaling-mediated osteoblast and adipocyte differentiation. TMEM64 interacts and positively regulates sarcoplasmic endoplasmic reticulum Ca(2+) ATPase 2 (SERCA2) activity, which is important for RANKL-induced Ca(2+) oscillation, Ca(2+)/calmodulin-dependent protein kinases (CaMK) IV activity and mitochondrial ROS formation needed for for osteoclast formation. Mice with Tmem64 deficiency are characterized by a high-bone-mass phenotype, displaying higher levels of ALP, osteocalcin (Ocn), and type 1 collagen (Col 1a) transcripts than in wild-type mice. Tmem64-knockout in mice is reported to markedly enhance osteoblast differentiation and inhibit adipocyte differentiation from bone marrow-derived stromal cells (BMSCs), resulting in a preferential switch of mesenchymal progenitors favoring the osteogenic over the adipogenic lineage.

~45 kDa observed. 39.67/12.95/14.25/33.97 kDa (human isoform 1/2/3/4) and 39.53 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human and rat TMEM64.

Detect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of SK-MEL-28 cell lysate.

The immunogen sequence is 100% conserved between human and rat species and present in all four human spliced isoforms reported by UniProt (Q6YI46 & D3ZV19). Murine cross-reactivity is highly likely, but has not been confirmed.

Format: Purified

Protein G purified.

Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of A431 cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.