conjugate
unconjugated
antibody form
purified antibody
clone
1E6, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
rat
General description
Oli-Neu cells (murine oligodendroglial precursor cells immortalized by an activated neu tyrosine kinase).
~330 kDa observed; 252.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated NG2 in immunoprecipitation applications (Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).
Western Blotting Analysis: A representative lot detected NG2 in Western Blotting applications (Stegmuller, J., et. al. (2002). J Neurocytol. 31(6-7):497-505; Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Trotter, J., et. al. (2005). J Neurol Sci. 233(1-2):37-42; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).
ELISA Analysis: A representative lot detected NG2 in ELISA applications (Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).
Immunofluorescence Analysis: A representative lot detected NG2 in Immunoprecipitation applications (Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Karram, K., et. al. (2005). J Anat. 207(6):735-44; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).
Immunohistochemistry Analysis: A representative lot detected NG2 in Immunohistochemistry applications (Karram, K., et. al. (2005). J Anat. 207(6):735-44; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61).
Western Blotting Analysis: A representative lot detected NG2 in Western Blotting applications (Stegmuller, J., et. al. (2002). J Neurocytol. 31(6-7):497-505; Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Trotter, J., et. al. (2005). J Neurol Sci. 233(1-2):37-42; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).
ELISA Analysis: A representative lot detected NG2 in ELISA applications (Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).
Immunofluorescence Analysis: A representative lot detected NG2 in Immunoprecipitation applications (Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Karram, K., et. al. (2005). J Anat. 207(6):735-44; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).
Immunohistochemistry Analysis: A representative lot detected NG2 in Immunohistochemistry applications (Karram, K., et. al. (2005). J Anat. 207(6):735-44; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61).
Biochem/physiol Actions
Clone 1E6 is a rat monoclonal antibody that detects mouse Chondroitin sulfate proteoglycan 4 (NG2).
Physical form
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected NG2 in mouse brain tissue lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected NG2 in mouse brain tissue lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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