Anti-NQO1, clone A180

Anti-NQO1, clone A180, Cat. No. MABS2176, is a mouse monoclonal antibody that detects NAD(P)H:Quinone Oxidoreductase 1 (NQO1) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation, and Western Blotting.

Immunocytochemistry Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting

Western Blotting Analysis: 1 µg/mL from a representative lot detected NQO1 in HepG2 and HeLa cell lysates.

Immunoprecipitation Analysis: A representative lot immunoprecipitated NQO1 in Immunoprecipitation applications (Siegel, D., et. al. (2018). PLoS One. 13(1):e0190717).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected NQO1 in Immunohistochemistry applications (Siegel, D., et. al. (2012). PLoS One. 7(9):e44861).

Immunocytochemistry Analysis: A representative lot detected NQO1 in Immunocytochemistry applications (Siegel, D., et. al. (2012). PLoS One. 7(9):e44861).

Western Blotting Analysis: A representative lot detected NQO1 in Western Blotting applications (Pink, J.J., et. al. (2000). J Biol Chem. 275(8):5416-24).

Clone A180 is a mouse monoclonal antibody that specifically detects human NAD(P)H dehydrogenase [quinone] 1 (NQO1).

Full-length recombinant human NAD(P)H dehydrogenase [quinone] 1 (NQO1).

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NQO1 in MCF7 cell lysate.

NAD(P)H dehydrogenase [quinone] 1 (UniProt: P15559; also known as EC:1.6.5.2, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, QR1, NQO1) is encoded by the NQO1 (also known as DIA4, NMOR1) gene (Gene ID: 1728) in human. NQO1 is a homodimeric FAD containing quinone reductase that catalyzes the 2-electron reduction of a broad range of quinones. It serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It can accept electrons from both NADH and NADPH with equal efficiency. Its activity can be induced by oxidative stress and offers protection against redox cycling and reactive oxygen species. It also protects a wide range of proteins, including p53, from proteasomal degradation. NQO1 is shown to be expressed at higher levels in epithelial tissues, vascular endothelium, and adipocytes. It is also expressed at higher levels in many solid tumors. (Ref.: Siegel, D., et al. (2012). PLoS ONE 7(9): e44861).

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