Anti-SIRP alpha Antibody, clone SAF10.1

Eukaryotically expressed SIRP-Fc fusion protein consisting of the extracellular domain.

~75 kDa observed; 54.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Western Blotting Analysis: 1 µg/mL from a representative lot detected SIRP alpha in A549 cell lysate.

Western Blotting Analysis: A represetntative lot detected SIRP alpha in Western Blotting applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).

ELISA Analysis: A represetntative lot detected SIRP alpha in ELISA applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).

Clone SAF10.1 is a mouse monoclonal antibody that detects human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRP alpha). It targets an epitope in the membrane most distal domain (D1).

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in U20S cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SIRP alpha in U20S cell lysate.

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