Anti-GRP-78 Antibody, clone C107

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

~72 kDa observed

Recombinant protein corresponding to human GRP-78.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

This Anti-GRP-78 Antibody, clone C107 is validated for use in western blotting, flow cytometry & Inhibits activity/Function for the detection of GRP-78.

Western Blotting Analysis: 1 µg/mL from a representative lot detected GRP-78 in 10 µg of HeLa cell lysate.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected GRP-78 in B16F1 cells from tumor bearing mice (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Activity Assay Analysis: A representative lot from an independent laboratory reduces tumor growth in mice with B16F1 melanoma cells (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected GRP-78 in 10 µg of HepG2 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.