Anti-GOT1 Antibody, clone 1F5.2

Aspartate aminotransferase, cytoplasmic (EC 2.6.2.1.; EC 2.6.2.3; UniProt P17174; also known as Aspartate aminotransferase 1, Aspartate transaminase 1, cAspAT, cCAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase cytoplasmic, Glutamate oxaloacetate transaminase 1, Growth-inhibiting protein 18, Transaminase A) is encoded by the GOT1 (also known as GIG18, ASTQTL1) gene (Gene ID 2805) in human. Aspartate aminotransferase is expressed in many types of cells and tissues and is conserved among prokaryotic and eukaryotic organisms. There exist two highly similar AST forms in mammals, the cytosolic cAST (encoded by the GOT1 gene) and the mitochondrial mAST (encoded by the GOT2 gene). Serum aspartate aminotransferase activity is largely that of cAST/GOT1, and elevated serum AST activity is associated with liver, heart, and muscle injury. AST catalyzes the reversible conversion between aspartate/alpha-ketoglutarate and oxaloacetate/glutamate, increasing oxaloacetate concentration therefore shifts the equilibrium of AST-catalzed reaction toward aspartate/alpha-ketoglutarate and away from glutatmate. Lowering blood glutamate concentration via oxaloacetate i.v. injection prompts an efflux of excess extracellular brain glutamate into the blood, which is reported to allevate the infarct size and edema in a rat model of ischemia induced by transient occlusion of the middle cerebral artery occlusion (MCAO).

~43 kDa observed

Epitope: N-terminal half

GST-tagged Recombinant protein corresponding to the N-terminal region of human GOT1.

Detect GOT1 using this mouse monoclonal Anti-GOT1 Antibody, clone 1F5.2, Cat. No. MABS508, validated for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GOT1 in human liver hepatocytes and in human cerebellum.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GOT1 in 10 µg of HepG2 cell lysate.

Concentration: Please refer to lot specific datasheet.

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