Anti-CYP7B1 Antibody, clone M17P3F2

25-hydroxycholesterol 7-alpha-hydroxylase (UniProt: O75881; also known as EC:1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase) is encoded by the CYP7B1 gene (Gene ID: 9420) in human. CYP7B1 is an enzyme involved in bile acid biosynthesis and shares 40% sequence identity to human cholesterol 7a-hydroxylase (CYP7A1). CYP7B1 localizes to the endoplasmic reticulum membrane and is expressed in brain, testis, ovary, prostate, liver, colon, kidney, and small intestine. It catalyzes the rate-limiting step in the conversion of cholesterol to bile acids. The classic pathway of bile acid synthesis is initiated by cholesterol 7alpha-hydroxylase in the liver and is tightly regulated by bile acid feedback, hormones, and diurnal rhythm. In contrast, the pathway initiated with sterol 27-hydroxylase is located mainly in extrahepatic tissues. An "alternative" pathway of bile acid synthesis begins with 27-hydroxylation of cholesterol by 27-hydroxylase (CYP27), followed by 7alpha-hydroxylation by oxysterol 7alpha-hydroxylase (CYP7B1). Mutations in CYP7B1 have been associated with Spastic paraplegia 5A, an autosomal recessive disorder that is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Disorders in CYP7B1 also cause congenital bile acid synthesis defect 3 (CBAS3) that leads to severe cholestasis, cirrhosis, and liver synthetic failure.

58.26 kDa calculated.

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal end of human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1).

Detect CYP7B1 using this mouse monoclonal Anti-CYP7B1 Antibody, clone M17P3F2, Cat. No. MABS513, used in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected CYP7B1 in human cerebral cortex and human pancreatic cancer tissue.

Immunohistochemistry Analysis: A representative lot detected CYP7B1 in in human liver tissue. (Courtesy of Ayham Alnabulsi, Ph.D., Vertebrate Antibodies Ltd. United Kingdom).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CYP7B1 in human liver tissue lysate.

Research Category
Signaling

Clone M17P3F2 detects CYP7B1 in human cerebral cortex, testis, pancreatic cancer, and colon cancer.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CYP7B1 in human testis tissue.

Concentration: Please refer to lot specific datasheet.

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