Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182.

Epitope: Phosphorylated Thr180 and Tyr182

Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182.

Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells.

Research Category
Signaling

Research Sub Category
MAP Kinases

Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.

41 kDa observed

Protein G Purified

Format: Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Anisomycin treated and untreated HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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