Anti-Keratan sulfate Antibody, clone 373E1

Keratan sulfate (also known as keratosulfate) is a widely distributed glycosaminoglycan in tensional and weight-bearing connective tissues, epithelial tissues, and the central and peripheral nervous system. It is attached to a number of proteoglycan and glycoprotein core proteins. Three types of keratan sulfate have been described: KS I, KS II, and KS III depending on their distribution pattern. Keratan sulfate is the only glycosaminoglycan (GAG) that does not contain uronic acid; therefore, it is not cleavable by the eliminative enzymatic cleavage. The biosynthesis of keratan sulfate occurs in two stages. In the first stage, the region binding the core protein with GAG is created followed by the elongation of the chain and its modification. The elongation of keratan sulfate chains of all types occurs by alternate attaching of Gal and GlcNAc residues, catalyzed by β-1,4-galactotransferase and β-1,3-N-acetyloglucosaminotransferase, respectively. Sulfation of keratan sulfate is carried out by at least two sulfotransferases. Gal 6-O-sulfotransferase transfers sulfate to position 6 of Gal residues located in the internal region of keratan sulfate and GlcNAc 6-O-sulfotransferase transfers sulfate to position 6 of GalNAc residues located at the nonreducing terminal of keratan sulfate. Clone 373E1 is a monoclonal antibody that detects a unique sialylation/ conformational-dependent KS epitope that detects a novel glycoprotein complex produced by papillary thyroid carcinoma cells. (Ref.: Magro, G., et al. (2003) Am. J. Pathol. 163(1); 183-196; Caterson, B., et al. (1982). J. Invest. Dermatol. 79 (suppl 1); 45-50; Coleman, PJ., et al. (1998). J. Physiol. 509(3); 695-710).

Purified proteoglycans from embryonic chicken.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human cartilage tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Keratan Sulfate in human cartilage tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected Keratan Sulfate in Western Blotting application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Keratan Sulfate in Immunoprecipitation application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Immunohistochemistry Applications: A representative lot detected Keratan Sulfate in Immunohistochemistry application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

ELISA Analysis: A representative lot detected Keratan Sulfate in ELISA application (Magro, G., et al. (2003). Am J Pathol. 163(1):183-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 373E1 is a mouse monoclonal antibody that detects Keratan sulfate in cartilage tissue.

Purified mouse monoclonal antibody IgM in PBS without preservatives.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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