Izumo sperm-egg fusion protein 1 (UniProt: Q9D9J7; also known as Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo) is encoded by the Izumo1 gene (Gene ID: 73456) in murine species. OBF is a single-pass type I membrane protein that is detected on sperm surface after the acrosome reaction. It is an essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. It can be present as a monomer, homodimer, or homooligomer. It is synthesized with a signal peptide (aa 1-21), which is cleaved off in the mature form, which has an extracellular domain (aa 22-319), a short transmembrane domain (aa 320-340), and a cytoplasmic domain (aa 341-397). The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Its interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation. A region comprising of 13 amino acids (148-160) is important for its interaction with IZUMO1R. Mab120 is a fusion-inhibiting antibody that inhibits fertilization. It binds to a region designated as putative functional fragment (aa 5-113). (Ref.: Inoue, N et al. (2013). Development. 140(15); 3221-3229).

~56 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Clone Mab120 is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 (IZUMO1) in murine species. It targets an epitope with in the N-terminal putative functional fragment.

Mouse spermatozoa.

Anti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting.

Inhibition Analysis: A representative lot inhibited fertilization in an in vitro system. (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Research Category
Cell Structure

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate.

Concentration: Please refer to lot specific datasheet.

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