Anti-MARVELD2/Tricellulin Antibody, clone N54

GST-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal region of murine Tricellulin.

~64 kDa observed; 63.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected MARVELD2/Tricellulin in mouse liver and kidney tissue sections.

Western Blotting Analysis: A representative lot detected MARVELD2/Tricellulin in Western Blotting applications (Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45).

Immunocytochemistry Analysis: A representative lot detected MARVELD2/Tricellulin in Immunocytochemistry applications (Kim, N.K., et. al. (2015). PLoS One. 10(2):e0116931).

Immunofluorescence Analysis: A representative lot detected MARVELD2/Tricellulin in Immunofluorescence applications (Ikenouchi, J., et. al. (2005). J Cell Biol. 171(6):939-45; Oda, Y., et. al. (2014). J Cell Sci. 127(Pt 19):4201-12; Yokouchi, M., et. al. (2016). Elife. 5. pii: e19593).

Clone N54 is a rat monoclonal antibody that specifically detects murine Tricellulin. It targets an epitope within the N-terminal cytoplasmic domain.

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in EpH4 cell lystate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MARVELD2/Tricellulin in EpH4 cell lystate.

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