Anti-ADAMTS5 Antibody, clone 3A2.1

101.7 kDa calculated

The ADAMTS (A Disintegrin and Metalloprotease with Thrombospondin motifs) family of secreted proteases comprises at least 20 members in mammals, and several members in invertebrates. ADAMTS members resemble the membrane-bound ADAM family of proteases in the presence of a prodomain, a catalytic domain and a disintegrin-like domain. However, ADAMTS members lack a transmembrane domain, and instead contain varying numbers of thrombospondin type I modules. ADAMTS-5, along with ADAMTS-4, cleaves aggrecan between Glu373 and Ala374, a physiological cleavage site in arthritic cartilage. In addition, expression of ADAMTS-5 is elevated in human chondrocytes treated with inflammatory cytokine interleukin-1, but is constitutively expressed in bovine cartilage explants. 70 and 53 kD forms of ADAMTS-5 have been shown to be present at varying ratios in fibroblast-like synoviocytes and extracts of rheumatoid arthritis, osteoarthritis and normal synovial tissue.

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human ADAMTS5.

Detect ADAMTS5 using this Anti-ADAMTS5 Antibody, clone 3A2.1 validated for use in IHC (Paraffin).

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunohistochemistry in human thalamus tissue.

Imunnohistochemistry Analysis: A 1:50 dilution of this antibody detected ADAMTS5 in human thalamus tissue.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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