Anti-Integrin B8 Antibody, clone11D4

Integrin beta-8 (UniProt: P26012) is encoded by the ITGB8 gene (Gene ID: 3696) in human. Integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. The integrin receptors are composed of alpha and beta subunits and each subunit crosses the membrane once, with most of the polypeptide residing in the extracellular space, and has two short cytoplasmic domains. Integrin beta-8 is a single-pass type I membrane protein that associates with alpha V chain in a non-covalent manner to make a heterodimer that serves as a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Integrin beta-8 binding to LAP is reported to promote the proteolytic release of active TGF-beta from LAP. Integrin beta-8 contains a VWFA domain and four cysteine-rich repeats. It is required for vascular morphogenesis in the embryonic brain and yolk sac. Higher expression of integrin beta-8 is observed in placenta, kidney, brain, ovary, uterus, and in several transformed cells. It is transiently expressed in 293 human embryonic kidney cells. Higher expression of ITGB8 is linked to high angiogenic and poorly invasive glioblastoma tumors. Conversely, low expression of ITGB8 correlates with highly invasive, but low angiogenic tumors. Higher expression of integrin beta-8 is observed in placenta, kidney, brain, ovary, uterus, and in several transformed cells. It is transiently expressed in 293 human embryonic kidney cells.

~71 kDa observed; 71.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

A truncated recombinant human Integrin B8, isoform 2, corresponding to amino acids 136-769.

Research Category
Cell Structure

This mouse monoclonal Anti-Integrin B8 Antibody, clone11D4, Cat. No. MABT912 is tested for use in Western Blotting for the detection of Integrin B8.

Clone11D4 specifically detects isoform 2 of human integrin beta-8.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Integrin B8 in 10 µg of HT-29 cell lysate.

Concentration: Please refer to lot specific datasheet.

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