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MBNMAG-41K

MILLIPLEX® Mouse Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteoprotegerin, SOST, TNF-α.

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UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

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species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 84-94%, standard curve range: 10-40,000 pg/mL
(Leptin), standard curve range: 15-60,000 pg/mL
(DKK-1), standard curve range: 16-65,000 pg/mL
(OPG), standard curve range: 3-12,000 pg/mL
(SOST), standard curve range: 3-12,000 pg/mL
(TNFα), standard curve range: 37-150,000 pg/mL
(Insulin), standard curve range: 4-15,000 pg/mL
(ACTH), standard curve range: 7-30,000 pg/mL
(FGF-23), standard curve range: 7-30,000 pg/mL
(IL-6)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Quality Level

200

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.

MILLIPLEX® Mouse Bone Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture supernatant samples: ACTH, IL-6, OPG, Insulin, Leptin, DKK1, SOST, TNFα and FGF23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Application

  • Analytes: ACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteoprotegerin (OPG), Sclerostin (SOST), TNF-α
  • Recommended Sample Type: Mouse Serum, plasma, cell/tissue culture supernatants or lysates
  • Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in appropriate control medium
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
  • Research Category: Bone
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

法规信息

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Jason Plummer et al.
Journal of cellular biochemistry, 118(1), 31-42 (2016-05-19)
Dietary methionine restriction (MR) increases longevity and improves healthspan in rodent models. Young male C57BL/6J mice were placed on MR to assess effects on bone structure and formation. Mice were fed diets containing 0.86% or 0.12% methionine for 5 weeks.
Amadou Ouattara et al.
Bone reports, 5, 33-42 (2016-01-01)
Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate
Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-?B activation.
Wu, K; Lin, TH; Liou, HC; Lu, DH; Chen, YR; Fu, WM; Yang, RS
Osteoporosis International : A Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA null
Positive environmental modification of depressive phenotype and abnormal hypothalamic-pituitary-adrenal axis activity in female C57BL/6J mice during abstinence from chronic ethanol consumption.
Pang, TY; Du, X; Catchlove, WA; Renoir, T; Lawrence, AJ; Hannan, AJ
Frontiers in Pharmacology null
Heather A Himburg et al.
Radiation research, 186(2), 141-152 (2016-07-09)
Ionizing radiation exposure can cause acute radiation sickness (ARS) by damaging the hematopoietic compartment. Radiation damages quiescent hematopoietic stem cells (HSCs) and proliferating hematopoietic cells, resulting in neutropenia, thrombocytopenia and increased risk for long-term hematopoietic dysfunction and myelodysplasia. While some
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Quality Control Ranges - Milliplex Mouse Bone Magnetic Bead Panel
Protocol- MBNMAG-41K
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